ERP27 Antibody / Endoplasmic reticulum resident protein 27 / TXNDC13

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

Q96DN0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ERP27 was used as the immunogen for the ERP27 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32E24

Applications

WB, IHC, ICC, IF

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This ERP27 antibody is available for research use only.

Storage Conditions

Store the ERP27 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the ERP27 antibody should be determined by the researcher.

Image Legend

Western blot testing of mouse pancreas tissue lysate with the ERP27 antibody at 1:2000 dilution for 1 hour at room temperature. A single band is detected at approximately 34 kDa, slightly above the predicted 30 kDa molecular weight. This shift is consistent with published data showing glycosylated, ER-localized forms of ERP27 migrating between 33-35 kDa. The observed band corresponds to the mature, post-translationally modified ERP27 protein.