GTF2E1 Antibody / TFIIE alpha

Product Specifications

Specifications

Immunofluorescence: 1:50-1:200, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Western blot: 1:500-1:2000

UniProt

P29083

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human TFIIE alpha was used as the immunogen for the GTF2E1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31G53

Applications

IF, IHC, ICC, WB

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This GTF2E1 antibody is available for research use only.

Storage Conditions

Store the GTF2E1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the GTF2E1 antibody should be determined by the researcher.

Image Legend

Western blot testing of human samples using the GTF2E1 antibody at 1:1000 dilution for 1 hour at room temperature. A dominant band is detected at ~60-65 kDa with a second band at ~50-55 kDa, although the calculated molecular weight is ~49 kDa. The higher-migrating species is consistent with hyper-phosphorylated TFIIE alpha during transcription initiation, while the lower band represents a less-phosphorylated form.