SOCS7 Antibody / Suppressor of cytokine signaling 7

Western blot analysis of SOCS7 using anti-SOCS7 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS7 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A band is detected in the commonly cited 58-63 kDa range. This migration is consistent with endogenous SOCS7 and reflects differences in phosphorylation state and isoform usage, which modestly alter SDS-PAGE mobility.