STAM2 Antibody / Signal transducing adaptor molecule 2

After reconstitution, the STAM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunofluorescent staining of STAM2 using anti-STAM2 antibody (red) and anti-Beta Tubulin antibody (green) . STAM2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-STAM2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight 488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. U2OS cells were stained with STAM2 antibody (red) and Beta-tubulin antibody (green) . STAM2 displays cytoplasmic punctate staining, concentrated in the perinuclear region consistent with its association with early/sorting endosomes. The vesicular STAM2 signal partially aligns with the microtubule network, reflecting its role in endosome trafficking along microtubule tracks.