GPNMB Antibody / Transmembrane glycoprotein NMB / HGFN

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

After reconstitution, the GPNMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunohistochemical staining of GPNMB using anti-GPNMB antibody. GPNMB was detected in paraffin-embedded section of human liver cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GPNMB antibody overnight at 4oC. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.