IL21R Antibody / Interleukin-21 receptor

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9HBE5

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human IL21R was used as the immunogen for the IL21R antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This IL21R antibody is available for research use only.

Storage Conditions

After reconstitution, the IL21R antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the IL21R antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Immunohistochemical staining of IL21R using anti-IL21R antibody. IL21R was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IL21R antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.