P4HA1 Antibody / Prolyl 4-hydroxylase subunit alpha 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P13674

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human P4HA1 was used as the immunogen for the P4HA1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, IF

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This P4HA1 antibody is available for research use only.

Storage Conditions

After reconstitution, the P4HA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the P4HA1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of P4HA1 using anti-P4HA1 antibody (red) . P4HA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-P4HA1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.