Cdh1 Antibody / E-cadherin
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
P09803
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708) was used as the immunogen for the Cdh1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, FACS, IF, IHC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This Cdh1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cdh1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the Cdh1 antibody should be determined by the researcher.
Image Legend
Immunohistochemical staining of E-Cadherin/Cdh1 using anti-Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Cdh1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
50772114 3x 1.0 µg
Zcchc9 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
Sign In for Pricing
View Details