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Cdh1 Antibody / E-cadherin

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

P09803

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708) was used as the immunogen for the Cdh1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IF, IHC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This Cdh1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cdh1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

http://www.nsjbio.com/tds-pdf/cdh1-antibody-e-cadherin-fy13118-nsj-bioreagents

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the Cdh1 antibody should be determined by the researcher.

Image Legend

Immunohistochemical staining of E-Cadherin/Cdh1 using anti-Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Cdh1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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