TIM50 Antibody / Translocase of inner mitochondrial membrane 50

Product Specifications

Specifications

Western blot: 1:500-1:2000

UniProt

Q3ZCQ8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human TIM50 was used as the immunogen for the TIM50 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32T72

Applications

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This TIM50 antibody is available for research use only.

Storage Conditions

Store the TIM50 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the TIM50 antibody should be determined by the researcher.

Image Legend

Western blot testing of mouse and rat samples using the TIM50 antibody at 1:1000 dilution for 1 hour at room temperature. TIM50 antibody detects a consistent band at ~36-37 kDa across multiple samples. Although the calculated mass is ~40 kDa, TIM50 undergoes mitochondrial presequence cleavage upon import, yielding a mature ~36 kDa form that predominates in western blot analyses of endogenous samples.