K27-linkage Specific Ubiquitin Antibody / UBB / UBC

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

P0CG47, P0CG48

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human K27-linkage Specific Ubiquitin was used as the immunogen for the K27-linkage Specific Ubiquitin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31U80

Applications

WB, IHC, ICC, IF

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This K27-linkage Specific Ubiquitin antibody is available for research use only.

Storage Conditions

Store the K27-linkage Specific Ubiquitin antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the K27-linkage Specific Ubiquitin antibody should be determined by the researcher.

Image Legend

Western blot analysis of K27-linkage Specific Ubiquitin expression in human HeLa cell lysate using K27-linkage Specific Ubiquitin antibody. A strong band near ~20 kDa in HeLa lysate is detected, corresponding to K27-linked di-ubiquitin. Although the UBB and UBC precursors have predicted masses of ~26 kDa and ~77 kDa, they are rapidly processed into monomeric and short polyubiquitin chains in cells; K27-linked Ub2 species are therefore the predominant form detected under standard conditions.