LIG4 Antibody / DNA Ligase 4

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P49917

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876) was used as the immunogen for the LIG4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This LIG4 antibody is available for research use only.

Storage Conditions

After reconstitution, the LIG4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the LIG4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of DNA Ligase IV/LIG4 using anti-LIG4 antibody (green) and anti-Tubulin Alpha antibody (red) . DNA Ligase IV/LIG4 was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-DNA Ligase IV/LIG4 antibody and mouse anti-Tubulin Alpha antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight 488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.