TNIP3 Antibody / TNFAIP3-interacting protein 3

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96KP6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human TNIP3 recombinant protein (Position: K30-P325) was used as the immunogen for the TNIP3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC/IF, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This TNIP3 antibody is available for research use only.

Storage Conditions

After reconstitution, the TNIP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the TNIP3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of TNIP3 using anti-TNIP3 antibody (green) . TNIP3 was detected in an immunocytochemical section of human U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TNIP3 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.