SMPD3 Antibody / Sphingomyelin phosphodiesterase 3 / nSMase2

After reconstitution, the SMPD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of SMPD3 using anti-SMPD3 antibody. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMPD3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. SMPD3 antibody detects the expected ~71 kDa monomer and an additional band near ~210 kDa in K562 and Caco-2 lysates. The higher band likely represents an SDS-resistant oligomeric form of nSMase2.