Phospho-eIF4EBP1 (Thr70) Antibody / EIF4EBP1
Product Specifications
Specifications
Western blot: 1:500-1:2000
UniProt
Q13541
Host
Rabbit
Reactivity
Human
Immunogen
A synthesized peptide derived from human Phospho-eIF4EBP1 (T70) was used as the immunogen for the Phospho-eIF4EBP1 (Thr70) antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
32E48
Applications
WB
Purity
Affinity chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This Phospho-eIF4EBP1 (Thr70) antibody is available for research use only.
Storage Conditions
Store the Phospho-eIF4EBP1 (Thr70) antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Phospho-eIF4EBP1 (Thr70) antibody should be determined by the researcher.
Image Legend
Western blot analysis of Phospho-eIF4EBP1 using anti-Phospho-eIF4EBP1 (Thr70) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-eIF4EBP1 (Thr70) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of Phospho-eIF4EBP1 is ~17 kDa.
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