TGIF2 Antibody / TG-interacting factor 2

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

UniProt

Q9GZN2

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human TGIF2 recombinant protein (Position: M1-Q237) was used as the immunogen for the TGIF2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC/IF, IP, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This TGIF2 antibody is available for research use only.

Storage Conditions

After reconstitution, the TGIF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the TGIF2 antibody should be determined by the researcher.

Location

Nucleus, Centrosome

Image Legend

Immunofluorescent staining of TGIF2 using anti-TGIF2 antibody (green) and anti-Beta Tubulin antibody (red) . TGIF2 was detected in an immunocytochemical section of U2OS cells and shows the expected nucleus and centrosome staining. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TGIF2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.