NRF2 Antibody / Nuclear factor erythroid 2-related factor 2 / NFE2L2

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Western Blot: 0.25-0.5 µg/mL

UniProt

Q16236

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601) was used as the immunogen for the NRF2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This NRF2 antibody is available for research use only.

Storage Conditions

After reconstitution, the NRF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the NRF2 antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of human RT4 cells using anti-NRF2 antibody. Overlay histogram showing RT4 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRF2 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.