SURF6 Antibody / Surfeit locus protein 6

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

O75683

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human SURF6 recombinant protein (Position: M1-R216) was used as the immunogen for the SURF6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SURF6 antibody is available for research use only.

Storage Conditions

After reconstitution, the SURF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SURF6 antibody should be determined by the researcher.

Location

Nucleus, Nucleolus

Image Legend

Immunofluorescent staining of SURF6 using anti-SURF6 antibody (red) and anti-Beta Tubulin antibody (green) . SURF6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SURF6 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.