GALP Antibody / Galanin-like peptide

After reconstitution, the GALP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of GALP using anti-GALP antibody. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALP antibody at 0.25 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of GALP shows a single band at ~17 kDa in human samples and ~15 kDa in mouse/rat, consistent with the small pro-peptide migrating slightly above the calculated ~13 kDa. An additional ~36 kDa band appears in mouse/rat liver only; given the low hepatic expression of GALP, this band likely reflects non-specific reactivity or an SDS-resistant aggregate rather than authentic GALP.