DNMT3A Antibody / DNA (cytosine-5) -methyltransferase 3A

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9Y6K1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human DNMT3A recombinant protein (Position: M1-Y284) was used as the immunogen for the DNMT3A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC, IF, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This DNMT3A antibody is available for research use only.

Storage Conditions

After reconstitution, the DNMT3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the DNMT3A antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunohistochemical staining of DNMT3A using anti-DNMT3A antibody. DNMT3A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-DNMT3A antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.