RPN1 Antibody / Ribophorin I

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Flow Cytometry: 1:50

UniProt

P04843

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A synthesized peptide derived from human Ribophorin I was used as the immunogen for the RPN1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32R32

Applications

WB, IHC, ICC, IF, FACS

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This RPN1 antibody is available for research use only.

Storage Conditions

Store the RPN1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the RPN1 antibody should be determined by the researcher.

Image Legend

Western blot testing of human samples using the RPN1 antibody at 1:3000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 68 kDa in all samples, consistent with the mature N-glycosylated ER membrane protein ribophorin I described in the literature. Additional weaker bands between roughly 50 and 60 kDa, likely represent truncated or processed RPN1 species generated during normal ER associated degradation rather than distinct full length isoforms.