COL2A1 Antibody / Collagen alpha-1 (II) chain

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P02458

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241) was used as the immunogen for the COL2A1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This COL2A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the COL2A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the COL2A1 antibody should be determined by the researcher.

Image Legend

Immunohistochemical staining of Collagen Type II/COL2A1 using anti-COL2A1 antibody. Collagen Type II/COL2A1 was detected in a paraffin-embedded section of mouse knee cartilage tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COL2A1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.