RASSF8 Antibody / Ras association domain-containing protein 8

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q8NHQ8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

E.coli-derived human RASSF8 recombinant protein (Position: E50-V419) was used as the immunogen for the RASSF8 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC, IF, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This RASSF8 antibody is available for research use only.

Storage Conditions

After reconstitution, the RASSF8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the RASSF8 antibody should be determined by the researcher.

Location

Cytoplasm, Mitochondria

Image Legend

Immunofluorescent staining of RASSF8 using anti-RASSF8 antibody (red) . RASSF8 was detected in an immunocytochemical section of human HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-RASSF8 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.