SLC15A5 Antibody / Solute carrier family 15 member 5

After reconstitution, the SLC15A5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of SLC15A5 using anti-SLC15A5 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC15A5 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A predominant band is detected at an approximately 70 kDa in all samples, running slightly above the predicted ~65 kDa size of the 579 amino acid SLC15A5 polypeptide, consistent with the higher apparent molecular weight expected for a glycosylated multi-pass membrane transporter.