MECP2 Antibody / Methyl-CpG-binding protein 2

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

P51608

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Immunogen

E.coli-derived human MECP2 recombinant protein (Position: K36-Q437) was used as the immunogen for the MECP2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This MECP2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MECP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the MECP2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of MECP2 using anti-MECP2 antibody (red) . MECP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-MECP2 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.