ALOX5AP Antibody / Arachidonate 5-lipoxygenase-activating protein

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P20292

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ALOX5AP was used as the immunogen for the ALOX5AP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC, IF, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This ALOX5AP antibody is available for research use only.

Storage Conditions

After reconstitution, the ALOX5AP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the ALOX5AP antibody should be determined by the researcher.

Location

ER, nuclear membrane

Image Legend

Immunofluorescent staining of ALOX5AP using anti-ALOX5AP antibody (green) . ALOX5AP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-ALOX5AP antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.