Ceacam1 Antibody / Carcinoembryonic antigen-related cell adhesion molecule 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P31809

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

E.coli-derived mouse Ceacam1 recombinant protein (Position: A34-D382) was used as the immunogen for the Ceacam1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This Ceacam1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ceacam1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the Ceacam1 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of CEACAM1 using anti-Ceacam1 antibody (green) . CEACAM1 was detected in an immunocytochemical section of mouse RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-Ceacam1 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.