IMPA1 Antibody / Inositol monophosphatase 1
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P29218
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human IMPA1 recombinant protein (Position: M1-D277) was used as the immunogen for the IMPA1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IP, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This IMPA1 antibody is available for research use only.
Storage Conditions
After reconstitution, the IMPA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the IMPA1 antibody should be determined by the researcher.
Image Legend
Immunoprecipitating IMPA1 in Jurkat whole cell lysate. Western blot analysis of IMPA1 using anti-IMPA1 antibody. Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-IMPA1 antibody in Jurkat whole cell lysate, Lane 3: anti-IMPA1 antibody (2ug) + Jurkat whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IMPA1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for IMPA1 at approximately 30 kDa. The expected molecular weight of IMPA1 is ~30 kDa.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog