SPA17 Antibody / Sperm autoantigenic protein 17
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
Q15506
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human SPA17 recombinant protein (Position: M1-K136) was used as the immunogen for the SPA17 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IF, IHC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SPA17 antibody is available for research use only.
Storage Conditions
After reconstitution, the SPA17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SPA17 antibody should be determined by the researcher.
Location
Cytoplasm (Golgi), cell memebrane
Image Legend
Immunofluorescent staining of SPA17 using anti-SPA17 antibody (green) . SPA17 was detected in a paraffin-embedded section of human ovarian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-SPA17 antibody overnight at 4oC. FITC Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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