SPA17 Antibody / Sperm autoantigenic protein 17

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q15506

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human SPA17 recombinant protein (Position: M1-K136) was used as the immunogen for the SPA17 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IF, IHC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SPA17 antibody is available for research use only.

Storage Conditions

After reconstitution, the SPA17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SPA17 antibody should be determined by the researcher.

Location

Cytoplasm (Golgi), cell memebrane

Image Legend

Immunofluorescent staining of SPA17 using anti-SPA17 antibody (green) . SPA17 was detected in a paraffin-embedded section of human ovarian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-SPA17 antibody overnight at 4oC. FITC Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.