DCAF13 Antibody / DDB1- and CUL4-associated factor 13

Western blot analysis of DCAF13 using anti-DCAF13 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF13 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected between approximately 45 and 48 kDa in all samples, running slightly below the predicted ~51 kDa mass but consistent with the apparent molecular weight of the processed nuclear WD repeat protein DCAF13.