DCAF13 Antibody / DDB1- and CUL4-associated factor 13

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50

UniProt

Q9NV06

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human DCAF13 was used as the immunogen for the DCAF13 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32D85

Applications

WB, IHC, IP

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This DCAF13 antibody is available for research use only.

Storage Conditions

Store the DCAF13 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the DCAF13 antibody should be determined by the researcher.

Image Legend

Western blot analysis of DCAF13 using anti-DCAF13 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF13 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected between approximately 45 and 48 kDa in all samples, running slightly below the predicted ~51 kDa mass but consistent with the apparent molecular weight of the processed nuclear WD repeat protein DCAF13.