TMEM232 Antibody / Transmembrane protein 232

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

C9JQI7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human TMEM232 recombinant protein (Position: D114-H598) was used as the immunogen for the TMEM232 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC/IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This TMEM232 antibody is available for research use only.

Storage Conditions

After reconstitution, the TMEM232 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the TMEM232 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of TMEM232 using anti-TMEM232 antibody (green) . TMEM232 was detected in an immunocytochemical section of human SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TMEM232 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.