GPR35 Antibody / G protein-coupled receptor 35

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9HC97

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GPR35 was used as the immunogen for the GPR35 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This GPR35 antibody is available for research use only.

Storage Conditions

After reconstitution, the GPR35 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the GPR35 antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of human HepG2 cells using anti-GPR35 antibody. Overlay histogram showing HepG2 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPR35 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.