MFSD4A Antibody / Major facilitator superfamily domain-containing protein 4A

After reconstitution, the MFSD4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of MFSD4A using anti-MFSD4A antibody. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFSD4A antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at an approximately 65 kDa in both samples, running above the predicted ~56 kDa mass but consistent with the higher apparent molecular weight typical of glycosylated multi pass membrane transporters such as MFSD4A.