RGL3 Antibody / Ral guanine nucleotide dissociation stimulator-like 3

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

After reconstitution, the RGL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunofluorescent staining of RGL3 using anti-RGL3 antibody (red) and anti-Beta Tubulin antibody (green) . RGL3 was detected in immunocytochemical section of human HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-RGL3 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. RGL3 shows nuclear localization in HeLa cells and cytoplasmic localization in human liver cancer tissue. Previous publicly available data suggest nucleoplasmic localization in some human cell lines (e.g., CACO-2, RT-4) and nuclear + cytoplasmic in another (U-2 OS) per proteinatlas.org, but comprehensive localization across cancer types is not yet established for RGL3.