OXSR1 Antibody / Oxidative stress-responsive kinase 1

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Western Blot: 0.25-0.5 µg/mL

UniProt

O95747

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human OXSR1 recombinant protein (Position: K351-S527) was used as the immunogen for the OXSR1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IP, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This OXSR1 antibody is available for research use only.

Storage Conditions

After reconstitution, the OXSR1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the OXSR1 antibody should be determined by the researcher.

Image Legend

Immunoprecipitating OXSR1 in human U251 whole cell lysate. Western blot analysis of OXSR1 using anti-OXSR1 antibody. Lane 1: U251 whole cell lysates (30ug) Lane 2: Rabbit control IgG instead of anti-OXSR1 antibody in U251 whole cell lysate. Lane 3: anti-OXSR1 antibody (2ug) + U251 whole cell lysate (500ug) After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OXSR1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected molecular weight of OXSR1 is at 58 kDa.