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TAF3 Antibody / TATA-box binding protein associated factor 3

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

Q5VWG9

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human TAF3 was used as the immunogen for the TAF3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

30T12

Applications

WB, IHC, ICC, IF

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This TAF3 antibody is available for research use only.

Storage Conditions

Store the TAF3 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the TAF3 antibody should be determined by the researcher.

Image Legend

Western blot analysis of TAF3 expression in human HeLa cell lysate using TAF2 antibody. TAF3 antibody detects a strong band at ~140-150 kDa. Although the theoretical mass is ~104 kDa, TAF3 (a TFIID subunit with long acidic/disordered regions) migrates markedly slower on SDS-PAGE, and phosphorylation further increases apparent size.
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