ROCK1 Antibody / Rho associated coiled coil containing protein kinase 1

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

UniProt

Q13464

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ROCK1 was used as the immunogen for the ROCK1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32R84

Applications

WB, IHC, IP, FACS

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This ROCK1 antibody is available for research use only.

Storage Conditions

Store the ROCK1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the ROCK1 antibody should be determined by the researcher.

Image Legend

Western blot testing of human samples using the ROCK1 antibody at 1:2000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 158 kDa in all samples, consistent with full length ROCK1. An additional weaker band is observed just above the 55 kDa marker, which likely represents a proteolytic fragment of ROCK1 generated during cell stress or sample preparation rather than a distinct isoform.