ROCK1 Antibody / Rho associated coiled coil containing protein kinase 1
Product Specifications
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50
UniProt
Q13464
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human ROCK1 was used as the immunogen for the ROCK1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
32R84
Applications
WB, IHC, IP, FACS
Purity
Affinity chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This ROCK1 antibody is available for research use only.
Storage Conditions
Store the ROCK1 antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the ROCK1 antibody should be determined by the researcher.
Image Legend
Western blot testing of human samples using the ROCK1 antibody at 1:2000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 158 kDa in all samples, consistent with full length ROCK1. An additional weaker band is observed just above the 55 kDa marker, which likely represents a proteolytic fragment of ROCK1 generated during cell stress or sample preparation rather than a distinct isoform.
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