Hsp70 Antibody / Heat shock protein 70 / HSPA8

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

UniProt

P0DMV8, P0DMV9

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Hsp70 was used as the immunogen for the Hsp70 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31H87

Applications

WB, IHC, ICC, IF, IP, FACS

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Hsp70 antibody is available for research use only.

Storage Conditions

Store the Hsp70 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Hsp70 antibody should be determined by the researcher.

Image Legend

Western blot analysis of Hsp70 using anti-Hsp70 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The predicted molecular weight of Hsp70 is ~70 kDa and ~64 kDa (two isoforms) .