Hsp70 Antibody / Heat shock protein 70 / HSPA8

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

Western blot analysis of Hsp70 using anti-Hsp70 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The predicted molecular weight of Hsp70 is ~70 kDa and ~64 kDa (two isoforms) .