TYMP Antibody / Thymidine phosphorylase / PD-ECGF

After reconstitution, the TYMP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunoprecipitating PD-ECGF/TYMP in SiHa whole cell lysate. Western blot analysis of PD-ECGF/TYMP using anti-TYMP antibody. Lane 1: SiHa whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TYMP antibody in SiHa whole cell lysate, Lane 3: anti-TYMP antibody (2ug) + SiHa whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TYMP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The input lane shows the characteristic doublet at approximately 50 kDa and in the high 40 kDa region. In contrast, the immunoprecipitated material displays a single band at approximately 50 kDa, indicating selective enrichment of the full length TYMP species. The control IgG lane shows no detectable signal, confirming specificity of the immunoprecipitation.