TMEM43 Antibody / Transmembrane protein 43 / LUMA

Western blot analysis of TMEM43 using anti-TMEM43 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM43 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at an approximately 40 kDa in all samples, slightly below the predicted ~45 kDa size but consistent with the apparent molecular weight typically observed for the integral membrane protein TMEM43 under denaturing conditions.