TMEM43 Antibody / Transmembrane protein 43 / LUMA

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

Q9BTV4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human TMEM43 was used as the immunogen for the TMEM43 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

30T30

Applications

WB, IHC

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This TMEM43 antibody is available for research use only.

Storage Conditions

Store the TMEM43 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the TMEM43 antibody should be determined by the researcher.

Image Legend

Western blot analysis of TMEM43 using anti-TMEM43 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM43 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at an approximately 40 kDa in all samples, slightly below the predicted ~45 kDa size but consistent with the apparent molecular weight typically observed for the integral membrane protein TMEM43 under denaturing conditions.