Phospho-RIP3 (Ser232) Antibody / RIPK3

Product Specifications

Specifications

Western blot: 1:500-1:2000

UniProt

Q9Y572

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-RIP3 (S232) was used as the immunogen for the Phospho-RIP3 (Ser232) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31R36

Applications

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-RIP3 (Ser232) antibody is available for research use only.

Storage Conditions

Store the Phospho-RIP3 (Ser232) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-RIP3 (Ser232) antibody should be determined by the researcher.

Image Legend

Western blot analysis of Phospho-RIP3 (S232) using anti-Phospho-RIP3 (S232) antibody. Lane 1: rat spleen tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-RIP3 (S232) antibody (S232) at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of Phospho-RIP3 (S232) is at 50 kDa.