Renin receptor Antibody / ATP6AP2

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells

UniProt

O75787

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Renin receptor/ATP6AP2 was used as the immunogen for the Renin receptor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC/IF, IP, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This Renin receptor antibody is available for research use only.

Storage Conditions

After reconstitution, the Renin receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the Renin receptor antibody should be determined by the researcher.

Location

Cytoplasm (ER, Lysosome)

Image Legend

Immunofluorescent staining of Renin receptor/ATP6AP2 using anti-Renin receptor antibody (green) . Renin receptor/ATP6AP2 was detected in an immunocytochemical section of human HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-Renin receptor antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.