EIF4A3 Antibody / Eukaryotic initiation factor 4A-III

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P38919

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human EIF4A3 recombinant protein (Position: R14-I411) was used as the immunogen for the EIF4A3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC/IF, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This EIF4A3 antibody is available for research use only.

Storage Conditions

After reconstitution, the EIF4A3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the EIF4A3 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of EIF4A3 using anti-EIF4A3 antibody (green) and anti-Beta Tubulin antibody (red) . EIF4A3 was detected in an immunocytochemical section of human A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-EIF4A3 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.