PCTP Antibody / Phosphatidylcholine transfer protein

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL

UniProt

P53808

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse PCTP was used as the immunogen for the PCTP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PCTP antibody is available for research use only.

Storage Conditions

After reconstitution, the PCTP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PCTP antibody should be determined by the researcher.

Image Legend

Western blot analysis of PCTP using anti-PCTP antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of PCTP is ~25 kDa.