STIM2 Antibody / Stromal interaction molecule 2
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
Q9P246
Host
Rabbit
Reactivity
Human, Rat
Immunogen
E.coli-derived human STIM2 recombinant protein (Position: L458-H726) was used as the immunogen for the STIM2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IF, ICC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This STIM2 antibody is available for research use only.
Storage Conditions
After reconstitution, the STIM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the STIM2 antibody should be determined by the researcher.
Location
Cytoplasm (ER)
Image Legend
Immunofluorescent staining of STIM2 using anti-STIM2 antibody (red) . STIM2 was detected in an immunocytochemical section of human HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-STIM2 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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