Involucrin Antibody

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Product Specifications

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL (formalin or acetone fixed tissues), Immunocytochemistry: Suitable

Host

Mouse

Reactivity

Human

Immunogen

Human involucrin was used as the immunogen for this Involucrin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

SY-5

Applications

WB, IHC-P, IHC-F, ICC

Purity

Ascites

Format

Ascites

Limitations

This Involucrin antibody is available for research use only.

Storage Conditions

After reconstitution, the Involucrin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Involucrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC staining of FFPE human lung cancer tissue with Involucrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.