Cytochrome C Antibody

Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunohistochemistry (Frozen) : 1-2 µg/mL, Immunocytochemistry: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P99999

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 91-105 (ERADLIAYLKKATNE) were used as the immunogen for this Cytochrome C antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Limitations

This Cytochrome C antibody is available for research use only.

Storage Conditions

After reconstitution, the Cytochrome C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Cytochrome C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of FFPE human Caco-2 cells with Cytochrome C antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.