B-Actin Antibody / Beta Actin Antibody

B-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P60709

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey, Zebrafish, Chicken

Immunogen

Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Limitations

This b-Actin antibody is available for research use only.

Storage Conditions

After reconstitution, the b-Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) zebrafish, 2) chicken heart, 3) chicken liver, 4) chicken brain, 5) monkey heart, 6) monkey lung, 7) monkey kidney and 8) monkey liver tissue lysate with b-Actin antibody. Predicted molecular weight ~48 kDa.